Abies alba

1) Description of study sites

  • German study sites are located in the Bavarian Forest National Park
  • We selected two sites located in old growth forest and marked 100 adult trees and 150 saplings (500 individuals in total).
  • Sites are located at 770 m and 1100 m a.s.l. 
  • Coordinates of sampling sites (WGS84/UTM)
    •  33 U 383292 5419773, 770 m
    • 33 U 382949 5425904, 1100 m
  • At each site, two data loggers were installed that record temperature and humidity at 15 min intervals.
  • Long term weather data comes from several local weather stations. Additionally, data for our plots was obtained from a statistical model which predicts the temperature, humidity, precipitation and global radiation for each sampling site, using independent variables extracted from results of geostatistical modelling with the digital terrain model ArcEGMO.

2) Genotyping methods:

  • Abies alba:
  1. All 500 individuals are genotyped at 232 SNPs on KASP platform (completed in March 2014)
  2. Resequencing of bud burst/bud set candidate genes (in progress) in order to develop a SNP assay for further candidate genes

3) Phenotypic traits

  • We obtained/will obtain the following data:
  1. Bud burst (done in 2014, planned for 2015)
  2. Dendroecological analysis (cores are currently being analysed with our partners at the WSL in Birmensdorf, Switzerland)
  3. LMA (planned for 2015)
  4. in adult trees: dbh
  5. in saplings: height, age, survival rate
  6. in the Botanical Garden in Marburg we currently monitor bud set in order to develop a protocol for phenotyping in our sites.